Effective clinical-scale production of dendritic cell vaccines by monocyte elutriation directly in medium, subsequent culture in bags and final antigen loading using …
M Erdmann, J Dörrie, N Schaft, E Strasser… - Journal of …, 2007 - journals.lww.com
Dendritic cell (DC) vaccination approaches are advancing fast into the clinic. The major
obstacle for further improvement is the current lack of a simple functionally “closed” system
to generate standardized monocyte-derived (mo) DC vaccines. Here, we significantly
optimized the use of the Elutra counterflow elutriation system to enrich monocytic DC
precursors by (1) developing an algorithm to avoid red blood cell debulking and associated
monocyte loss before elutriation, and (2) by elutriation directly in culture medium rather than …
obstacle for further improvement is the current lack of a simple functionally “closed” system
to generate standardized monocyte-derived (mo) DC vaccines. Here, we significantly
optimized the use of the Elutra counterflow elutriation system to enrich monocytic DC
precursors by (1) developing an algorithm to avoid red blood cell debulking and associated
monocyte loss before elutriation, and (2) by elutriation directly in culture medium rather than …