Background aims Mesenchymal stromal cells (MSCs) have been applied to patients in cell
therapy for various diseases. Recently, we introduced a novel MSC separation filter device
which could yield approximately 2.5-fold more MSCs from bone marrow in a closed system
compared with the conventional open density gradient centrifugation method. MSCs isolated
with these two methods were phenotypically similar and met the criteria defining human
MSC proposed by the International Society for Cellular Therapy. However, these criteria do …