Expression of the full set of human and mouse groups I, II, V, X, and XII secreted
phospholipases A 2 (sPLA 2 s) in Escherichia coli and insect cells has provided pure
recombinant enzymes for detailed comparative interfacial kinetic and binding studies. The
set of mammalian sPLA 2 s display dramatically different sensitivity to dithiothreitol. The
specific activity for the hydrolysis of vesicles of differing phospholipid composition by these
enzymes varies by up to 4 orders of magnitude, and yet all enzymes display similar catalytic …