[HTML][HTML] Cytosolic phospholipase A2 is required for cytokine-induced expression of type IIA secretory phospholipase A2 that mediates optimal cyclooxygenase-2 …

H Kuwata, Y Nakatani, M Murakami, I Kudo - Journal of Biological …, 1998 - ASBMB
Activation of rat fibroblastic 3Y1 cells with interleukin-1β (IL-1β) and tumor necrosis factor α
(TNFα) induced delayed prostaglandin (PG) E 2 generation over 6–48 h, which occurred in
parallel with de novo induction of type IIA secretory phospholipase A 2 (sPLA 2) and
cyclooxygenase (COX)-2, without accompanied by changes in the constitutive expression of
type IV cytosolic PLA 2 (cPLA 2) and COX-1. Types V and IIC sPLA 2 s were barely
detectable in these cells. Studies using an anti-type IIA sPLA 2 antibody, sPLA 2 inhibitors …