In vivo bioluminescence imaging in a rabbit model of orthopaedic implant-associated infection to monitor efficacy of an antibiotic-releasing coating
RJ Miller, JM Thompson, J Zheng, MC Marchitto… - JBJS, 2019 - journals.lww.com
JBJS, 2019•journals.lww.com
Background: In vivo bioluminescence imaging (BLI) provides noninvasive monitoring of
bacterial burden in animal models of orthopaedic implant-associated infection (OIAI).
However, technical limitations have limited its use to mouse and rat models of OIAI. The goal
of this study was to develop a larger, rabbit model of OIAI using in vivo BLI to evaluate the
efficacy of an antibiotic-releasing implant coating. Methods: A nanofiber coating loaded with
or without linezolid-rifampin was electrospun onto a surgical-grade locking peg. To model …
bacterial burden in animal models of orthopaedic implant-associated infection (OIAI).
However, technical limitations have limited its use to mouse and rat models of OIAI. The goal
of this study was to develop a larger, rabbit model of OIAI using in vivo BLI to evaluate the
efficacy of an antibiotic-releasing implant coating. Methods: A nanofiber coating loaded with
or without linezolid-rifampin was electrospun onto a surgical-grade locking peg. To model …
Abstract
Background:
In vivo bioluminescence imaging (BLI) provides noninvasive monitoring of bacterial burden in animal models of orthopaedic implant-associated infection (OIAI). However, technical limitations have limited its use to mouse and rat models of OIAI. The goal of this study was to develop a larger, rabbit model of OIAI using in vivo BLI to evaluate the efficacy of an antibiotic-releasing implant coating.
Methods:
A nanofiber coating loaded with or without linezolid-rifampin was electrospun onto a surgical-grade locking peg. To model OIAI in rabbits, a medial parapatellar arthrotomy was performed to ream the femoral canal, and a bright bioluminescent methicillin-resistant Staphylococcus aureus (MRSA) strain was inoculated into the canal, followed by retrograde insertion of the coated implant flush with the articular surface. In vivo BLI signals were confirmed by ex vivo colony-forming units (CFUs) from tissue, bone, and implant specimens.
Results:
Lippincott Williams & Wilkins