Targeting LncRNAs in cardiovascular disease: options and expeditions
S Haemmig, MW Feinberg - Circulation research, 2017 - Am Heart Assoc
S Haemmig, MW Feinberg
Circulation research, 2017•Am Heart Assoccomplicates silencing of a single lncRNA. Morrbid promoter deletion was achieved using 2
single guide RNAs targeting the 5′ and 3′ flanking region of its promoter. Single guide
RNAs were cloned into Cas9 vector containing either a GFP (green fluorescent protein) or
mCherry selection marker, which were subsequently used for sorting of double-positive cells
(Online Figure IC). 2 The primary challenge that remains is the rather low efficiency of
homozygote knockout clones, although deletion frequency is inversely related to deletion …
single guide RNAs targeting the 5′ and 3′ flanking region of its promoter. Single guide
RNAs were cloned into Cas9 vector containing either a GFP (green fluorescent protein) or
mCherry selection marker, which were subsequently used for sorting of double-positive cells
(Online Figure IC). 2 The primary challenge that remains is the rather low efficiency of
homozygote knockout clones, although deletion frequency is inversely related to deletion …
complicates silencing of a single lncRNA. Morrbid promoter deletion was achieved using 2 single guide RNAs targeting the 5′ and 3′ flanking region of its promoter. Single guide RNAs were cloned into Cas9 vector containing either a GFP (green fluorescent protein) or mCherry selection marker, which were subsequently used for sorting of double-positive cells (Online Figure IC). 2 The primary challenge that remains is the rather low efficiency of homozygote knockout clones, although deletion frequency is inversely related to deletion size.
Am Heart Assoc