A fit-for-purpose LC–MS/MS method for the simultaneous quantitation of ATP and 2, 3-DPG in human K2EDTA whole blood
H Kim, P Kosinski, C Kung, L Dang, Y Chen… - … of Chromatography B, 2017 - Elsevier
Many hemolytic anemias results in major metabolic abnormalities: two common metabolite
abnormalities include increased levels of 2, 3-diphosphoglycerate (2, 3-DPG) and
decreased levels of adenosine triphosphate (ATP). To better monitor the concentration
changes of these metabolites, the development of a reliable LC–MS/MS method to
quantitatively profile the concentrations of 2, 3-DPG and ATP in whole blood is essential to
understand the effects of investigational therapeutics. Accurate quantification of both …
abnormalities include increased levels of 2, 3-diphosphoglycerate (2, 3-DPG) and
decreased levels of adenosine triphosphate (ATP). To better monitor the concentration
changes of these metabolites, the development of a reliable LC–MS/MS method to
quantitatively profile the concentrations of 2, 3-DPG and ATP in whole blood is essential to
understand the effects of investigational therapeutics. Accurate quantification of both …