Lysine acetylation targets protein complexes and co-regulates major cellular functions

C Choudhary, C Kumar, F Gnad, ML Nielsen… - Science, 2009 - science.org
Lysine acetylation is a reversible posttranslational modification of proteins and plays a key
role in regulating gene expression. Technological limitations have so far prevented a global
analysis of lysine acetylation's cellular roles. We used high-resolution mass spectrometry to
identify 3600 lysine acetylation sites on 1750 proteins and quantified acetylation changes in
response to the deacetylase inhibitors suberoylanilide hydroxamic acid and MS-275. Lysine
acetylation preferentially targets large macromolecular complexes involved in diverse …
Lysine acetylation is a reversible posttranslational modification of proteins and plays a key role in regulating gene expression. Technological limitations have so far prevented a global analysis of lysine acetylation’s cellular roles. We used high-resolution mass spectrometry to identify 3600 lysine acetylation sites on 1750 proteins and quantified acetylation changes in response to the deacetylase inhibitors suberoylanilide hydroxamic acid and MS-275. Lysine acetylation preferentially targets large macromolecular complexes involved in diverse cellular processes, such as chromatin remodeling, cell cycle, splicing, nuclear transport, and actin nucleation. Acetylation impaired phosphorylation-dependent interactions of 14-3-3 and regulated the yeast cyclin-dependent kinase Cdc28. Our data demonstrate that the regulatory scope of lysine acetylation is broad and comparable with that of other major posttranslational modifications.
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