Detection of true IgE-expressing mouse B lineage cells
MP Gallagher, A Shrestha, JM Magee… - JoVE (Journal of …, 2014 - jove.com
B lymphocyte immunoglobulin heavy chain (IgH) class switch recombination (CSR) is a
process wherein initially expressed IgM switches to other IgH isotypes, such as IgA, IgE and
IgG. Measurement of IgH CSR in vitro is a key method for the study of a number of biologic
processes ranging from DNA recombination and repair to aspects of molecular and cellular
immunology. In vitro CSR assay involves the flow cytometric measurement surface Ig
expression on activated B cells. While measurement of IgA and IgG subclasses is …
process wherein initially expressed IgM switches to other IgH isotypes, such as IgA, IgE and
IgG. Measurement of IgH CSR in vitro is a key method for the study of a number of biologic
processes ranging from DNA recombination and repair to aspects of molecular and cellular
immunology. In vitro CSR assay involves the flow cytometric measurement surface Ig
expression on activated B cells. While measurement of IgA and IgG subclasses is …