Horizontal whole mount: a novel processing and imaging protocol for thick, three-dimensional tissue cross-sections of skin
L Salz, RR Driskell - JoVE (Journal of Visualized Experiments), 2017 - jove.com
Processing a tissue of interest to generate a microscopic image that supports a scientific
argument can be challenging. The acquisition of high-quality microscopic images is not
entirely dependent upon the quality of the microscope, but also upon the methods of tissue
processing, which often involve multiple critical actions or steps. Furthermore, mesenchymal
cell types in the skin and other tissues represent a new challenge for tissue preparation and
imaging. Here, we present a complete process, from tissue harvest to microscopy. Our …
argument can be challenging. The acquisition of high-quality microscopic images is not
entirely dependent upon the quality of the microscope, but also upon the methods of tissue
processing, which often involve multiple critical actions or steps. Furthermore, mesenchymal
cell types in the skin and other tissues represent a new challenge for tissue preparation and
imaging. Here, we present a complete process, from tissue harvest to microscopy. Our …