Addendum: a reproducible and well-tolerated method for 2/3 partial hepatectomy in mice

C Mitchell, H Willenbring - Nature protocols, 2014 - nature.com
C Mitchell, H Willenbring
Nature protocols, 2014nature.com
In this addendum we provide an update to the part of our protocol 'A reproducible and well-
tolerated method for 2/3 partial hepatectomy in mice'that is related to analgesia1. Since the
publication of this protocol in 2008, researchers' awareness of and commitment to reducing
postsurgical pain in mice have increased markedly, and the use of analgesics should now
be the norm. In addition, experimental data are now available about efficacy and toxicity of
analgesics in mice subjected to 2/3 partial hepatectomy (2/3 PH) 2. Below we provide …
In this addendum we provide an update to the part of our protocol ‘A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice’that is related to analgesia1. Since the publication of this protocol in 2008, researchers’ awareness of and commitment to reducing postsurgical pain in mice have increased markedly, and the use of analgesics should now be the norm. In addition, experimental data are now available about efficacy and toxicity of analgesics in mice subjected to 2/3 partial hepatectomy (2/3 PH) 2. Below we provide modifications to be made to the procedure to achieve effective analgesia without aggravating liver injury in mice undergoing 2/3 PH. In addition, we reiterate and extend our recommendations regarding choice of anesthetic and control of body temperature.
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