Human vascular endothelial cells produce tumor necrosis factor-α in response to proinflammatory cytokine stimulation

V Ranta, A Orpana, O Carpén, U Turpeinen… - Critical care …, 1999 - journals.lww.com
Objective: To determine whether human vascular endothelial cells produce tumor necrosis
factor-α (TNF-α) after stimulation with proinflammatory cytokines and bacterial
lipopolysaccharides (LPS). Design: Prospective, in vitro repeated-measurements analysis of
cellular responses. Setting: Research laboratory in an academic medical center. Subjects:
Human umbilical vein endothelial cells (HUVECs). Interventions: HUVECs were incubated
with interferon-γ (IFN-γ), interleukin-1β (IL-1β), and LPS, or their different combinations for 2 …