Insulinotropic glucagon-like peptide I receptor expression in glucagon-producing α-cells of the rat endocrine pancreas
RS Heller, TJ Kieffer, JF Habener - Diabetes, 1997 - Am Diabetes Assoc
RS Heller, TJ Kieffer, JF Habener
Diabetes, 1997•Am Diabetes AssocRESEARCH DESIGN AND METHODS Isolation of islets. Pancreatic islets were isolated by a
modification of the original procedure of Lacy and Kostianovsky (16) from male and female
Sprague Dawley rats weighing between 250 and 400 g. Rats were anesthetized with
chloroform, and a median incision on the ventral surface of the abdomen exposed the
pancreas. The common bile duct was ligated where it empties into the duodenum, then
cannulated and injected with 10 ml of RPMI-1640 (Gibco Laboratories, Grand Island, NY) …
modification of the original procedure of Lacy and Kostianovsky (16) from male and female
Sprague Dawley rats weighing between 250 and 400 g. Rats were anesthetized with
chloroform, and a median incision on the ventral surface of the abdomen exposed the
pancreas. The common bile duct was ligated where it empties into the duodenum, then
cannulated and injected with 10 ml of RPMI-1640 (Gibco Laboratories, Grand Island, NY) …
RESEARCH DESIGN AND METHODS
Isolation of islets. Pancreatic islets were isolated by a modification of the original procedure of Lacy and Kostianovsky (16) from male and female Sprague Dawley rats weighing between 250 and 400 g. Rats were anesthetized with chloroform, and a median incision on the ventral surface of the abdomen exposed the pancreas. The common bile duct was ligated where it empties into the duodenum, then cannulated and injected with 10 ml of RPMI-1640 (Gibco Laboratories, Grand Island, NY) containing 1 mg/ml collagenase P (Boehringer
Am Diabetes Assoc