Effects of the cardiomyopathy-causing E244D mutation of troponin T on the structures of cardiac thin filaments studied by small-angle X-ray scattering
T Matsuo, F Kono, S Fujiwara - Journal of structural biology, 2019 - Elsevier
Small-angle X-ray scattering experiments were carried out to investigate the structural
changes of cardiac thin filaments induced by the cardiomyopathy-causing E244D mutation
in troponin T (TnT). We examined native thin filaments (NTF) from a bovine heart,
reconstituted thin filaments containing human cardiac wild-type Tn (WTF), and filaments
containing the E244D mutant of Tn (DTF), in the absence and presence of Ca 2+. Analysis
by model calculation showed that upon Ca 2+-activation, tropomyosin (Tm) and Tn in the …
changes of cardiac thin filaments induced by the cardiomyopathy-causing E244D mutation
in troponin T (TnT). We examined native thin filaments (NTF) from a bovine heart,
reconstituted thin filaments containing human cardiac wild-type Tn (WTF), and filaments
containing the E244D mutant of Tn (DTF), in the absence and presence of Ca 2+. Analysis
by model calculation showed that upon Ca 2+-activation, tropomyosin (Tm) and Tn in the …