Capture-ELISA based on recombinant PR3 is sensitive for PR3–ANCA testing and allows detection of PR3 and PR3–ANCA/PR3 immunecomplexes
J Sun, DN Fass, JA Hudson, MA Viss… - Journal of …, 1998 - Elsevier
Proteinase 3 (PR3), a constituent of azurophil granules of neutrophils (polymorphonuclear
cells, PMNs), is the target antigen for most anti-neutrophil cytoplasmic antibodies (c-ANCA)
in Wegener's granulomatosis (WG). We have recently developed an expression system for
recombinant PR3 (rPR3) that is recognized by c-ANCA. Here, we report on the development
and characterization of two monoclonal antibodies (moABs) and a rabbit polyclonal
antiserum generated against this rPR3. Epitope competition analysis indicates that the …
cells, PMNs), is the target antigen for most anti-neutrophil cytoplasmic antibodies (c-ANCA)
in Wegener's granulomatosis (WG). We have recently developed an expression system for
recombinant PR3 (rPR3) that is recognized by c-ANCA. Here, we report on the development
and characterization of two monoclonal antibodies (moABs) and a rabbit polyclonal
antiserum generated against this rPR3. Epitope competition analysis indicates that the …