[HTML][HTML] Tryptase and protease-activated receptor 2 expression levels in irritable bowel syndrome
WJ Liang, G Zhang, HS Luo, LX Liang, D Huang… - Gut and liver, 2016 - ncbi.nlm.nih.gov
WJ Liang, G Zhang, HS Luo, LX Liang, D Huang, FC Zhang
Gut and liver, 2016•ncbi.nlm.nih.govMethods Colonoscopic biopsies were performed of 38 subjects (20 with IBS-diarrhea [IBS-
D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein
levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels
of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide
(CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue
staining. Results Significant increases in the mRNA expression of tryptase (p< 0.05, IBS-D …
D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein
levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels
of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide
(CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue
staining. Results Significant increases in the mRNA expression of tryptase (p< 0.05, IBS-D …
Methods
Colonoscopic biopsies were performed of 38 subjects (20 with IBS-diarrhea [IBS-D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue staining.
Results
Significant increases in the mRNA expression of tryptase (p< 0.05, IBS-D, IBS-C vs control) and PAR-2 (p< 0.05, IBS-D, IBS-C vs control) and in the tryptase protein level (p< 0.05, IBS-D, IBS-C vs control) were detected in IBS. Elevations of MCs, CGRP, VIP and SP (p< 0.05, IBS-D vs control) were observed for IBS-D only.
Conclusions
Tryptase levels may upregulate the function of PAR-2, resulting in the release of neuropeptide and they were correlated with clinical symptoms associated with IBS.
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