A single injection of gain-of-function mutant PCSK9 adeno-associated virus vector induces cardiovascular calcification in mice with no genetic modification
C Goettsch, JD Hutcheson, S Hagita, MA Rogers… - Atherosclerosis, 2016 - Elsevier
Background and aims Studying atherosclerotic calcification in vivo requires mouse models
with genetic modifications. Previous studies showed that injection of recombinant adeno-
associated virus vector (AAV) encoding a gain-of-function mutant PCSK9 into mice promotes
atherosclerosis. We aimed to study cardiovascular calcification induced by PCSK9 AAV in
C57BL/6J mice. Methods 10 week-old C57BL/6J mice received a single injection of AAV
encoding mutant mPCSK9 (rAAV8/D377Y-mPCSK9). Ldlr–/–mice served as positive …
with genetic modifications. Previous studies showed that injection of recombinant adeno-
associated virus vector (AAV) encoding a gain-of-function mutant PCSK9 into mice promotes
atherosclerosis. We aimed to study cardiovascular calcification induced by PCSK9 AAV in
C57BL/6J mice. Methods 10 week-old C57BL/6J mice received a single injection of AAV
encoding mutant mPCSK9 (rAAV8/D377Y-mPCSK9). Ldlr–/–mice served as positive …