[HTML][HTML] Glucagon-like peptide-1 receptor signalling selectively regulates murine lymphocyte proliferation and maintenance of peripheral regulatory T cells

I Hadjiyanni, KA Siminovitch, JS Danska, DJ Drucker - Diabetologia, 2010 - Springer
I Hadjiyanni, KA Siminovitch, JS Danska, DJ Drucker
Diabetologia, 2010Springer
Aims/hypothesis Glucagon-like peptide-1 receptor (GLP-1R) agonists improve glucose
control in animals and humans with type 1 diabetes. However, there is little information on
the role of the GLP-1R in the immune system. We studied the role of the GLP-1R in immune
function in wild-type (WT) and non-obese diabetic (NOD) and Glp1r−/− mice. Methods Glp1r
mRNA expression was examined in sorted immune subpopulations by RT–PCR. The effects
of GLP-1R activation were assessed on cAMP production and proliferation, migration and …
Aims/hypothesis
Glucagon-like peptide-1 receptor (GLP-1R) agonists improve glucose control in animals and humans with type 1 diabetes. However, there is little information on the role of the GLP-1R in the immune system. We studied the role of the GLP-1R in immune function in wild-type (WT) and non-obese diabetic (NOD) and Glp1r −/− mice.
Methods
Glp1r mRNA expression was examined in sorted immune subpopulations by RT–PCR. The effects of GLP-1R activation were assessed on cAMP production and proliferation, migration and survival of primary immune cells from WT and NOD mice. The ability of primary cells from Glp1r −/− mice to proliferate, migrate or survive apoptosis was determined. Immunophenotyping studies were performed to assess the frequency of immune subpopulations in Glp1r −/− mice.
Results
Ex vivo activation of the GLP-1R resulted in a modest but significant elevation of cAMP in primary thymocytes and splenocytes from both WT and NOD mice. GLP-1R activation did not increase proliferation of primary thymocytes, splenocytes or peripheral lymph node cells. In contrast, Glp1r −/− thymocytes exhibited a hypoproliferative response, whilst peripheral Glp1r −/− lymphocytes were hyperproliferative in response to mitogenic stimulation. Activation or loss of GLP-1R signalling did not modify apoptosis or chemotaxis in primary lymphocytes. Male Glp1r −/− mice exhibited a significantly lower percentage of peripheral regulatory T cells, although no differences were observed in the numbers of CD4+ and CD8+ T cells and B cells in the spleen and lymph nodes of Glp1r −/− mice.
Conclusions/interpretation
These studies establish that GLP-1R signalling may regulate lymphocyte proliferation and maintenance of peripheral regulatory T cells.
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