Defective FcγRIIb1 signaling contributes to enhanced calcium response in B cells from patients with systemic lupus erythematosus
EJ Enyedy, JP Mitchell, MP Nambiar, GC Tsokos - Clinical immunology, 2001 - Elsevier
B lymphocytes from patients with systemic lupus erythematosus (SLE) display enhanced B
cell antigen receptor (BCR)-mediated early signal transduction events, including increased
fluxes of intracytoplasmic calcium ([Ca2+] i). Because crosslinking of FcγRIIb1 (CD32) in
normal B cells suppresses the BCR-initiated signal transduction process, we investigated
whether the increased BCR-initiated [Ca2+] i response in SLE B cells is the consequence of
decreased FcγRIIb1-mediated suppression. To this end, we used flow cytometry to study the …
cell antigen receptor (BCR)-mediated early signal transduction events, including increased
fluxes of intracytoplasmic calcium ([Ca2+] i). Because crosslinking of FcγRIIb1 (CD32) in
normal B cells suppresses the BCR-initiated signal transduction process, we investigated
whether the increased BCR-initiated [Ca2+] i response in SLE B cells is the consequence of
decreased FcγRIIb1-mediated suppression. To this end, we used flow cytometry to study the …
