Non‐technical summary  The human biological clock organizes and regulates the timing of many biochemical and physiological processes, including the timing of sleep, on a daily basis. Light is the strongest time cue to the circadian clock that keeps these rhythms entrained to the 24 h day. Light exposure at night results in ‘resetting’ of the clock (phase shifting). In the current study, we examined the effects of exposing subjects to two different light levels (very dim light vs. typical room light) before exposure to a 6.5 h light exposure at night. Results showed that the very dim light level, compared to the typical room light level, prior to the light exposure at night caused a substantially greater phase shift of the melatonin rhythm and substantially greater acute melatonin suppression. Thus, prior dim light history sensitizes the human biological clock to the effect of a subsequent light exposure.