[HTML][HTML] Growth and Differentiation Proceeds Normally in Cells Deficient in the Immediate Early Gene NGFI-A (∗)
SL Lee, LAC Tourtellotte, RL Wesselschmidt… - Journal of Biological …, 1995 - ASBMB
NGFI-A (also known as EGR-1, zif/268, and Krox-24) is a zinc finger transcription factor
induced in many cell types by a variety of growth and differentiation stimuli. To determine if
NGFI-A plays a requisite role in these processes, we used homologous recombination to
mutate both alleles of NGFI-A in embryonic stem (ES) cells and examined its effect on
growth and differentiation. We find that ES cells lacking NGFI-A exhibit similar growth rates
and serum-induced gene expression profiles compared to wild-type parental cells. They are …
induced in many cell types by a variety of growth and differentiation stimuli. To determine if
NGFI-A plays a requisite role in these processes, we used homologous recombination to
mutate both alleles of NGFI-A in embryonic stem (ES) cells and examined its effect on
growth and differentiation. We find that ES cells lacking NGFI-A exhibit similar growth rates
and serum-induced gene expression profiles compared to wild-type parental cells. They are …