Neurons exhibit Lyz2 promoter activity in vivo: Implications for using LysM-Cre mice in myeloid cell research.
J Orthgiess, M Gericke, K Immig… - European journal of …, 2016 - search.ebscohost.com
J Orthgiess, M Gericke, K Immig, A Schulz, J Hirrlinger, I Bechmann, J Eilers
European journal of immunology, 2016•search.ebscohost.comAbstract To characterize LysM‐Cre mediated gene targeting in mice, we crossed LysM‐Cre
mice to two independent reporter‐mouse lines (tdTomato or YFP). Surprisingly, we found
that more than 90% of cells with LysM‐Cre mediated recombination in the brain were
neurons, rather than myeloid cells, such as microglia. Hence, by using the LysM‐Cre mouse
line for conditional knockout approaches, a significant neuronal recombination needs to be
considered.
mice to two independent reporter‐mouse lines (tdTomato or YFP). Surprisingly, we found
that more than 90% of cells with LysM‐Cre mediated recombination in the brain were
neurons, rather than myeloid cells, such as microglia. Hence, by using the LysM‐Cre mouse
line for conditional knockout approaches, a significant neuronal recombination needs to be
considered.
Abstract To characterize LysM‐Cre mediated gene targeting in mice, we crossed LysM‐Cre mice to two independent reporter‐mouse lines (tdTomato or YFP). Surprisingly, we found that more than 90% of cells with LysM‐Cre mediated recombination in the brain were neurons, rather than myeloid cells, such as microglia. Hence, by using the LysM‐Cre mouse line for conditional knockout approaches, a significant neuronal recombination needs to be considered.
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