Background We previously showed that the presence of a CKD-associated locus in
SHROOM3 in a donor kidney results in increased expression of SHROOM3 (an F-actin–
binding protein important for epithelial morphogenesis, via rho-kinase [ROCK] binding); this
facilitates TGF-b signaling and allograft fibrosis. However, other evidence suggests
Shroom3 may have a protective role in glomerular development. Methods We used human
data, Shroom3 knockdown podocytes, and inducible shRNA-mediated knockdown mice to …