[HTML][HTML] A lipidomic screen of hyperglycemia-treated HRECs links 12/15-Lipoxygenase to microvascular dysfunction during diabetic retinopathy via NADPH oxidase
AS Ibrahim, S Elshafey, H Sellak, KA Hussein… - Journal of Lipid …, 2015 - ASBMB
Retinal hyperpermeability and subsequent macular edema is a cardinal feature of early
diabetic retinopathy (DR). Here, we investigated the role of bioactive lipid metabolites, in
particular 12/15-lipoxygenase (LOX)-derived metabolites, in this process. LC/MS lipidomic
screen of human retinal endothelial cells (HRECs) demonstrated that 15-HETE was the only
significantly increased metabolite (2.4±0.4-fold, P= 0.0004) by high glucose (30 mM)
treatment. In the presence of arachidonic acid, additional eicosanoids generated by 12/15 …
diabetic retinopathy (DR). Here, we investigated the role of bioactive lipid metabolites, in
particular 12/15-lipoxygenase (LOX)-derived metabolites, in this process. LC/MS lipidomic
screen of human retinal endothelial cells (HRECs) demonstrated that 15-HETE was the only
significantly increased metabolite (2.4±0.4-fold, P= 0.0004) by high glucose (30 mM)
treatment. In the presence of arachidonic acid, additional eicosanoids generated by 12/15 …