The use of cryoEM and three-dimensional image reconstruction is becoming increasingly
common. Our vision for this technique is to provide a straightforward manner in which users
can proceed from raw data to a reliable 3D reconstruction through a pipeline that both
facilitates management of the processing steps and makes the results at each step more
transparent. Tightly integrated with a relational SQL database, Appion is a modular and
transparent pipeline that extends existing software applications and procedures. The user …