Derailed B cell homeostasis in patients with mixed connective tissue disease
A Hajas, S Barath, P Szodoray, B Nakken, P Gogolak… - Human immunology, 2013 - Elsevier
Mixed connective tissue disease (MCTD) is a systemic autoimmune disorder, characterized
by the presence of antibodies to U1-RNP protein. We aimed to determine phenotypic
abnormalities of peripheral B cell subsets in MCTD. Blood samples were obtained from 46
MCTD patients, and 20 controls. Using anti-CD19, anti-CD27, anti-IgD and anti-CD38
monoclonal antibodies, the following B cell subsets were identified by flow cytometry:(1)
transitional B cells (CD19+ CD27-IgD+ CD38high);(2) naive B cells (CD19+ CD27-IgD+ …
by the presence of antibodies to U1-RNP protein. We aimed to determine phenotypic
abnormalities of peripheral B cell subsets in MCTD. Blood samples were obtained from 46
MCTD patients, and 20 controls. Using anti-CD19, anti-CD27, anti-IgD and anti-CD38
monoclonal antibodies, the following B cell subsets were identified by flow cytometry:(1)
transitional B cells (CD19+ CD27-IgD+ CD38high);(2) naive B cells (CD19+ CD27-IgD+ …