T-helper signals restore B-cell receptor signaling in autoreactive anergic B cells by upregulating CD45 phosphatase activity
Background We recently identified a human B-cell population that is naturally autoreactive
and tolerized by functional anergy (B ND cells). Objective We sought to identify the
molecular mechanism of how anergic autoreactive B ND cells escape functional anergy and
whether this process is altered in patients with lupus. Methods Isolated peripheral blood
naive and B ND cells were cultured with various stimuli, and their activation status was
determined by using an intracellular Ca 2+ mobilization assay. Lyn kinase and Syk activities …
and tolerized by functional anergy (B ND cells). Objective We sought to identify the
molecular mechanism of how anergic autoreactive B ND cells escape functional anergy and
whether this process is altered in patients with lupus. Methods Isolated peripheral blood
naive and B ND cells were cultured with various stimuli, and their activation status was
determined by using an intracellular Ca 2+ mobilization assay. Lyn kinase and Syk activities …