Selective recruitment of polarized T cells expressing CCR5 and CXCR3 to the inflamed joints of children with juvenile idiopathic arthritis
LR Wedderburn, N Robinson, A Patel… - … : Official Journal of …, 2000 - Wiley Online Library
LR Wedderburn, N Robinson, A Patel, H Varsani, P Woo
Arthritis & Rheumatism: Official Journal of the American College …, 2000•Wiley Online LibraryObjective To study the expression of chemokine receptors CCR5 and CXCR3 and the
Th1/Th2 cytokine balance in children with oligoarticular or polyarticular juvenile idiopathic
arthritis (JIA). Methods Using 3‐color immunofluorescence, we studied the expression of
CCR5 and CXCR3 on, and T cell cytokine production by, paired samples of synovial fluid
(SF) and peripheral blood (PB) T cells from 20 patients with oligoarticular‐or polyarticular‐
onset JIA. Chemokine and cytokine phenotypes were also compared within the CD45RO+ …
Th1/Th2 cytokine balance in children with oligoarticular or polyarticular juvenile idiopathic
arthritis (JIA). Methods Using 3‐color immunofluorescence, we studied the expression of
CCR5 and CXCR3 on, and T cell cytokine production by, paired samples of synovial fluid
(SF) and peripheral blood (PB) T cells from 20 patients with oligoarticular‐or polyarticular‐
onset JIA. Chemokine and cytokine phenotypes were also compared within the CD45RO+ …
Objective
To study the expression of chemokine receptors CCR5 and CXCR3 and the Th1/Th2 cytokine balance in children with oligoarticular or polyarticular juvenile idiopathic arthritis (JIA).
Methods
Using 3‐color immunofluorescence, we studied the expression of CCR5 and CXCR3 on, and T cell cytokine production by, paired samples of synovial fluid (SF) and peripheral blood (PB) T cells from 20 patients with oligoarticular‐ or polyarticular‐onset JIA. Chemokine and cytokine phenotypes were also compared within the CD45RO+,CD3+ subsets. CCR5 genotypes were confirmed by polymerase chain reaction typing and sequencing.
Results
In the majority of samples, the number of T cells that were CCR5+ and CXCR3+ was higher in SF than in PB, and this difference was significant. One child was homozygous for the null Δ32 CCR5 allele; 4 others had lower expression of CXCR3 in SF than in blood. All samples showed strongly Th1‐type cytokine production by synovial T cells compared with that by PB T cells. Both features were also markedly polarized within the synovial CD45RO+ subset compared with PB CD45RO+ T cells.
Conclusion
The high expression of CCR5 and CXCR3 and high interferon‐γ:interleukin‐4 ratios suggest a type 1 phenotype of SF T cells in JIA. The difference between CD45RO+ T cells from SF and from PB suggests that specific activation events have occurred in synovial T cells. We suggest that the highly activated, Th1‐type phenotype of T cells within the chronically inflamed joints of children with JIA may reflect specific recruitment events that contribute to the polarization of these cells.
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