[HTML][HTML] Opposing effects of oxygen regulation on kallistatin expression: kallistatin as a novel mediator of oxygen-induced HIF-1-eNOS-NO pathway

J Chao, Y Guo, P Li, L Chao - Oxidative medicine and cellular …, 2017 - hindawi.com
J Chao, Y Guo, P Li, L Chao
Oxidative medicine and cellular longevity, 2017hindawi.com
Oxidative stress has both detrimental and beneficial effects. Kallistatin, a key component of
circulation, protects against vascular and organ injury. Serum kallistatin levels are reduced
in patients and animal models with hypertension, diabetes, obesity, and cancer. Reduction
of kallistatin levels is inversely associated with elevated thiobarbituric acid-reactive
substance. Kallistatin therapy attenuates oxidative stress and increases endothelial nitric
oxide synthase (eNOS) and NO levels in animal models. However, kallistatin administration …
Oxidative stress has both detrimental and beneficial effects. Kallistatin, a key component of circulation, protects against vascular and organ injury. Serum kallistatin levels are reduced in patients and animal models with hypertension, diabetes, obesity, and cancer. Reduction of kallistatin levels is inversely associated with elevated thiobarbituric acid-reactive substance. Kallistatin therapy attenuates oxidative stress and increases endothelial nitric oxide synthase (eNOS) and NO levels in animal models. However, kallistatin administration increases reactive oxygen species formation in immune cells and bacterial killing activity in septic mice. High oxygen inhibits kallistatin expression via activating the JNK-FOXO1 pathway in endothelial cells. Conversely, mild oxygen/hyperoxia stimulates kallistatin, eNOS, and hypoxia-inducible factor-1 (HIF-1) expression in endothelial cells and in the kidney of normal mice. Likewise, kallistatin stimulates eNOS and HIF-1, and kallistatin antisense RNA abolishes oxygen-induced eNOS and HIF-1 expression, indicating a role of kallistatin in mediating mild oxygen’s stimulation on antioxidant genes. Protein kinase C (PKC) activation mediates HIF-1-induced eNOS synthesis in response to hyperoxia/exercise; thus, mild oxygen through PKC activation stimulates kallistatin-mediated HIF-1 and eNOS synthesis. In summary, oxidative stress induces down- or upregulation of kallistatin expression, depending on oxygen concentration, and kallistatin plays a novel role in mediating oxygen/exercise-induced HIF-1-eNOS-NO pathway.
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