[HTML][HTML] Reduction in liver fat by dietary MUFA in type 2 diabetes is helped by enhanced hepatic fat oxidation

L Bozzetto, G Costabile, D Luongo, D Naviglio… - Diabetologia, 2016 - Springer
L Bozzetto, G Costabile, D Luongo, D Naviglio, V Cicala, C Piantadosi, L Patti, P Cipriano…
Diabetologia, 2016Springer
Aims/hypothesis The aim of this work was to investigate hepatic lipid metabolic processes
possibly involved in the reduction of liver fat content (LF) observed in patients with type 2
diabetes after an isoenergetic diet enriched in monounsaturated fatty acids (MUFAs).
Methods This is an ancillary analysis of a published study. In a parallel-group design, 30
men and eight women, aged 35–70 years, with type 2 diabetes and whose blood glucose
was controlled satisfactorily (HbA 1c< 7.5%[58 mmol/mol]) by diet or diet plus metformin …
Aims/hypothesis
The aim of this work was to investigate hepatic lipid metabolic processes possibly involved in the reduction of liver fat content (LF) observed in patients with type 2 diabetes after an isoenergetic diet enriched in monounsaturated fatty acids (MUFAs).
Methods
This is an ancillary analysis of a published study. In a parallel-group design, 30 men and eight women, aged 35–70 years, with type 2 diabetes and whose blood glucose was controlled satisfactorily (HbA1c < 7.5% [58 mmol/mol]) by diet or diet plus metformin, were randomised by MINIM software to follow either a high-carbohydrate/high-fibre/low-glycaemic index diet (CHO/fibre diet, n = 20) or a high-MUFA diet (MUFA diet, n = 18) for 8 weeks. The assigned diets were known for the participants and blinded for people doing measurements. Before and after intervention, LF was measured by 1H-MRS (primary outcome) and indirect indices of de novo lipogenesis (DNL) (serum triacylglycerol palmitic:linoleic acid ratio), stearoyl-CoA desaturase activity (SCD-1) (serum triacylglycerol palmitoleic:palmitic acid ratio) and hepatic β-oxidation of fatty acids (β-hydroxybutyrate plasma concentrations) were measured.
Results
LF was reduced by 30% after the MUFA diet, as already reported. Postprandial β-hydroxybutyrate incremental AUC (iAUC) was significantly less suppressed after the MUFA diet (n = 16) (−2504 ± 4488 μmol/l × 360 min vs baseline −9021 ± 6489 μmol/l × 360 min) while it was unchanged after the CHO/fibre diet (n = 17) (−8168 ± 9827 μmol/l × 360 min vs baseline −7206 ± 10,005 μmol/l × 360 min, p = 0.962) (mean ± SD, p = 0.043). In the participants assigned to the MUFA diet, the change in postprandial β-hydroxybutyrate iAUC was inversely associated with the change in LF (r = −0.642, p = 0.010). DNL and SCD-1 indirect indices did not change significantly after either of the dietary interventions.
Conclusions/interpretation
Postprandial hepatic oxidation of fatty acids is a metabolic process possibly involved in the reduction of LF by a MUFA-rich diet in patients with type 2 diabetes.
Trial registration
ClinicalTrials.gov NCT01025856
Funding
The study was funded by Ministero Istruzione Università e Ricerca and Italian Minister of Health.
Springer