High intake of dietary fat plays an important role in obesity development in animals and humans, and prolonged intake of high‐fat diet might lead to low‐grade chronic inflammation. Previous study showed that diet‐induced overweight delays cutaneous wound healing in both obesity‐prone and obesity‐resistant animals, highlighting the importance of diet composition in the wound healing process. This study evaluated the hypothesis that a short‐term administration of high‐fat diet could affect cutaneous wound healing. Male mice (C57/bl6) were randomly divided into standard (10% energy from fat) or high‐fat (60% energy from fat) chow groups. After 10 days of diet administration, an excisional lesion was performed and the animals were sacrificed 6 or 10 days later. There was no difference in the fasting blood glucose between groups. Ten days after wounding, high‐fat chow group presented increased inflammatory infiltrate, levels of inducible nitric oxide synthase and cyclo‐oxygenase‐2 proteins, and lipid peroxidation. The high‐fat chow group presented delayed wound closure, increased amount of myofibroblasts and vessels, and decreased deposition of type I collagen. These findings support the hypothesis that short‐term administration of high‐fat diet exerts negative effects on mice cutaneous wound healing, due to the interference in the inflammatory phase.