[HTML][HTML] Estrogen-induced activation of Cdk4 and Cdk2 during G1-S phase progression is accompanied by increased cyclin D1 expression and decreased cyclin …

OWJ Prall, B Sarcevic, EA Musgrove, CKW Watts… - Journal of Biological …, 1997 - ASBMB
Estrogens induce cell proliferation in target tissues by stimulating progression through G 1
phase of the cell cycle, but the underlying molecular targets remain undefined. To determine
the role of the cyclin/cyclin-dependent kinase (CDK)/retinoblastoma protein (pRB) pathway
in this response we treated MCF-7 breast cancer cells with the pure estrogen antagonist ICI
182780 to inhibit estrogen-induced gene expression and induce G 1 phase arrest.
Subsequent treatment with 17β-estradiol resulted in the synchronous entry of cells into S …