A highly sensitive assay of human plasma xanthine oxidoreductase activity using stable isotope-labeled xanthine and LC/TQMS
T Murase, M Nampei, M Oka, A Miyachi… - … of Chromatography B, 2016 - Elsevier
Associations between elevated plasma xanthine oxidoreductase (XOR) activity and various
pathologies have been widely reported. However, it has been difficult to accurately measure
human plasma XOR activity because the XOR activity of humans is lower than that of
animals such as mouse. We developed a highly sensitive assay for XOR activity utilizing a
combination of [13 C 2, 15 N 2] xanthine and liquid chromatography/triple quadrupole mass
spectrometry. In the present study, we established and validated a novel human plasma …
pathologies have been widely reported. However, it has been difficult to accurately measure
human plasma XOR activity because the XOR activity of humans is lower than that of
animals such as mouse. We developed a highly sensitive assay for XOR activity utilizing a
combination of [13 C 2, 15 N 2] xanthine and liquid chromatography/triple quadrupole mass
spectrometry. In the present study, we established and validated a novel human plasma …