[HTML][HTML] Binding of xanthine oxidase to vascular endothelium: kinetic characterization and oxidative impairment of nitric oxide-dependent signaling

M Houston, A Estevez, P Chumley, M Aslan… - Journal of Biological …, 1999 - ASBMB
Concentrations of up to 1.5 milliunits/ml xanthine oxidase (XO)(1.1 μg/ml) are found
circulating in plasma during diverse inflammatory events. The saturable, high affinity binding
of extracellular XO to vascular endothelium and the effects of cell binding on both XO
catalytic activity and differentiated vascular cell function are reported herein. Xanthine
oxidase purified from bovine cream bound specifically and with high affinity (K d= 6 nm) at 4°
C to bovine aortic endothelial cells, increasing cell XO specific activity up to 10-fold …