Transactivation of sphingosine 1-phosphate receptors is essential for vascular barrier regulation: novel role for hyaluronan and CD44 receptor family
PA Singleton, SM Dudek, SF Ma, JGN Garcia - Journal of Biological …, 2006 - jbc.org
The role for hyaluronan (HA) and CD44 in vascular barrier regulation is unknown. We
examined high and low molecular weight HA (HMW-HA,∼ 1,000 kDa; LMW-HA,∼ 2.5 kDa)
effects on human transendothelial monolayer electrical resistance (TER). HMW-HA
increased TER, whereas LMW-HA induced biphasic TER changes ultimately resulting in EC
barrier disruption. HMW-HA induced the association of the CD44s isoform with, and AKT-
mediated phosphorylation of, the barrier-promoting sphingosine 1-phosphate receptor (S1P …
examined high and low molecular weight HA (HMW-HA,∼ 1,000 kDa; LMW-HA,∼ 2.5 kDa)
effects on human transendothelial monolayer electrical resistance (TER). HMW-HA
increased TER, whereas LMW-HA induced biphasic TER changes ultimately resulting in EC
barrier disruption. HMW-HA induced the association of the CD44s isoform with, and AKT-
mediated phosphorylation of, the barrier-promoting sphingosine 1-phosphate receptor (S1P …
