Isolation and culture of murine heart and lung endothelial cells for in vitro model systems
YC Lim, FW Luscinskas - Cell-Cell Interactions: Methods and Protocols, 2006 - Springer
YC Lim, FW Luscinskas
Cell-Cell Interactions: Methods and Protocols, 2006•SpringerThe inflammatory response is a critical component of host defense. An important goal of our
group has been to understand the endothelial-dependent mechanisms that mediate
leukocyte recruitment during an inflammatory response. In this chapter, we present a
detailed method for the isolation and in vitro culture of murine vascular endothelial cells from
the lung and heart. The endothelial cells are of high purity (85–99%) and retain certain of
their functional differences, including constitutive and cytokine inducible adhesion molecule …
group has been to understand the endothelial-dependent mechanisms that mediate
leukocyte recruitment during an inflammatory response. In this chapter, we present a
detailed method for the isolation and in vitro culture of murine vascular endothelial cells from
the lung and heart. The endothelial cells are of high purity (85–99%) and retain certain of
their functional differences, including constitutive and cytokine inducible adhesion molecule …
Abstract
The inflammatory response is a critical component of host defense. An important goal of our group has been to understand the endothelial-dependent mechanisms that mediate leukocyte recruitment during an inflammatory response. In this chapter, we present a detailed method for the isolation and in vitro culture of murine vascular endothelial cells from the lung and heart. The endothelial cells are of high purity (85–99%) and retain certain of their functional differences, including constitutive and cytokine inducible adhesion molecule expression and chemokine production. Such endothelial cells, therefore, can be used for various in vitro models including leukocyte adhesion assay or in depth biochemical analyses.
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