Efficient laboratory-scale production of monoclonal antibodies using membrane-based high-density cell culture technology
M Trebak, JM Chong, D Herlyn, DW Speicher - Journal of immunological …, 1999 - Elsevier
Monoclonal antibodies (MAbs) are important tools used in basic research as well as in the
imaging and therapy of cancer. Many countries have limited the use of animals for large-
scale production of MAbs, obliging laboratories to find efficient in vitro alternatives to ascites
production. In this report we describe a protocol for laboratory-scale production of MAbs by
culturing hybridoma cells in the two-chamber cell culture device CELLine 1000. This culture
flask supports high cell densities (107–108 cells/ml) and generates high concentrations of …
imaging and therapy of cancer. Many countries have limited the use of animals for large-
scale production of MAbs, obliging laboratories to find efficient in vitro alternatives to ascites
production. In this report we describe a protocol for laboratory-scale production of MAbs by
culturing hybridoma cells in the two-chamber cell culture device CELLine 1000. This culture
flask supports high cell densities (107–108 cells/ml) and generates high concentrations of …