Increased exosome production from tumour cell cultures using the Integra CELLine Culture System
JP Mitchell, J Court, MD Mason, Z Tabi… - Journal of immunological …, 2008 - Elsevier
Exosomes are nanometer-sized vesicles, secreted from most cell types, with documented
immune-modulatory functions. Exosomes can be purified from cultured cells but to do so
effectively, requires maintenance of cells at high density in order to obtain sufficient
accumulation of exosomes in the culture medium, prior to purification. Whilst high density
cultures can be achieved with cells in suspension, this remains difficult with adherent cells,
resulting in low quantity of exosomes for subsequent study. We have used the Integra …
immune-modulatory functions. Exosomes can be purified from cultured cells but to do so
effectively, requires maintenance of cells at high density in order to obtain sufficient
accumulation of exosomes in the culture medium, prior to purification. Whilst high density
cultures can be achieved with cells in suspension, this remains difficult with adherent cells,
resulting in low quantity of exosomes for subsequent study. We have used the Integra …