Efficient major histocompatibility complex class I presentation of exogenous antigen upon phagocytosis by macrophages.

M Kovacsovics-Bankowski, K Clark… - Proceedings of the …, 1993 - National Acad Sciences
M Kovacsovics-Bankowski, K Clark, B Benacerraf, KL Rock
Proceedings of the National Academy of Sciences, 1993National Acad Sciences
Antigens in extracellular fluids can be processed and presented with major
histocompatibility complex (MHC) class I molecules by a subset of antigen presenting cells
(APCs). Chicken egg ovalbumin (Ova) linked to beads was presented with MHC class I
molecules by these cells up to 10 (4)-fold more efficiently than soluble Ova. This enhanced
presentation was observed with covalently or noncovalently linked Ova and with beads of
different compositions. A key parameter in the activity of these conjugates was the size of the …
Antigens in extracellular fluids can be processed and presented with major histocompatibility complex (MHC) class I molecules by a subset of antigen presenting cells (APCs). Chicken egg ovalbumin (Ova) linked to beads was presented with MHC class I molecules by these cells up to 10(4)-fold more efficiently than soluble Ova. This enhanced presentation was observed with covalently or noncovalently linked Ova and with beads of different compositions. A key parameter in the activity of these conjugates was the size of the beads. The APC that is responsible for this form of presentation is a macrophage. These cells internalize the antigen constructs through phagocytosis, since cytochalasin B inhibited presentation. Processing of the antigen and association with MHC class I molecules appears to occur intracellularly as presentation was observed under conditions where there was no detectable release of peptides into the extracellular fluids. When injected in vivo in C57BL/6 mice, Ova-beads, but not soluble Ova, primed CD4- CD8+ cytotoxic T lymphocytes (CTLs). Similar results were obtained in BALB/c mice immunized with beta-galactosidase-beads. The implications of these findings for development of nonliving vaccines that stimulate CTL immunity are discussed.
National Acad Sciences