Recent advances in live cell imaging allow investigating processes that take place over the
entire cell volume with unprecedented time and spatial resolution. Here we describe a
protocol to study intercellular communication, including extracellular vesicle exchange,
between cancer cells and their microenvironment, using lattice light sheet fluorescence
microscopy. While the described protocol is intended to study the interactions between
chronic lymphocytic leukemia cells and bone marrow stromal cells, many components of it …