Isolation of mouse respiratory epithelial cells and exposure to experimental cigarette smoke at air liquid interface

HC Lam, AMK Choi, SW Ryter - JoVE (Journal of Visualized Experiments), 2011 - jove.com
Pulmonary epithelial cells can be isolated from the respiratory tract of mice and cultured at
air-liquid interface (ALI) as a model of differentiated respiratory epithelium. A protocol is
described for isolating and exposing these cells to mainstream cigarette smoke (CS), in
order to study epithelial cell responses to CS exposure. The protocol consists of three parts:
the isolation of airway epithelial cells from mouse trachea, the culturing of these cells at air-
liquid interface (ALI) as fully differentiated epithelial cells, and the delivery of calibrated …