Associations of innate and adaptive immune cell subsets with incident type 2 diabetes risk: the MESA study
NC Olson, MF Doyle, CM Sitlani… - The Journal of …, 2020 - academic.oup.com
The Journal of Clinical Endocrinology & Metabolism, 2020•academic.oup.com
Objective Cell-mediated immunity is implicated in glucose homeostasis and insulin
resistance. Whether the levels of innate and adaptive immune cells in peripheral blood are
risk factors for incident type 2 diabetes (T2D) remains unknown. We hypothesized that the
proportions of naive, memory, CD28−, Th17, and T regulatory CD4+ cells would be
associated with incident T2D. In secondary analyses, we evaluated the relationships of 28
additional immune cell phenotypes with T2D. Design Immune cell phenotypes (n= 33) were …
resistance. Whether the levels of innate and adaptive immune cells in peripheral blood are
risk factors for incident type 2 diabetes (T2D) remains unknown. We hypothesized that the
proportions of naive, memory, CD28−, Th17, and T regulatory CD4+ cells would be
associated with incident T2D. In secondary analyses, we evaluated the relationships of 28
additional immune cell phenotypes with T2D. Design Immune cell phenotypes (n= 33) were …
Objective
Cell-mediated immunity is implicated in glucose homeostasis and insulin resistance. Whether the levels of innate and adaptive immune cells in peripheral blood are risk factors for incident type 2 diabetes (T2D) remains unknown. We hypothesized that the proportions of naive, memory, CD28−, Th17, and T regulatory CD4+ cells would be associated with incident T2D. In secondary analyses, we evaluated the relationships of 28 additional immune cell phenotypes with T2D.
Design
Immune cell phenotypes (n = 33) were measured by flow cytometry using cryopreserved cells collected from 1113 participants of the Multi-Ethnic Study of Atherosclerosis (MESA) at the baseline examination (2000–2002). Cox proportional hazards models were used to evaluate associations of immune cell phenotypes with incident T2D over a median follow-up of 9.1 years, adjusted for age, sex, race/ethnicity, educational status, and body mass index.
Results
Incident T2D was observed for 120 participants. None of the cell phenotypes included in the primary hypotheses were significantly associated with T2D (all P > 0.05). Among the secondary immune cells studied, a higher proportion of CD19+CD27+ B cells was associated with a reduced risk of T2D (hazard ratio: 0.72 (95% confidence interval: 0.56, 0.93), per 1-standard deviation (16%) increase). This association was no longer significant after correction for the multiple cell phenotypes tested (P > 0.0015).
Conclusions
Our results suggest that the frequencies of several subsets of monocytes, innate lymphocytes, and CD4+ and CD8+ T cells in circulating blood are not related to the future onset of T2D. Higher levels of CD19+CD27+ B cells may be associated with decreased T2D risk.
Oxford University Press