[HTML][HTML] Folding of the amino-terminal domain of apolipoprotein B initiates microsomal triglyceride transfer protein-dependent lipid transfer to nascent very low density …

MF Ingram, GS Shelness - Journal of Biological Chemistry, 1997 - ASBMB
The initial assembly of apolipoprotein B100 (apoB) into lipoprotein particles occurs
cotranslationally. To examine steps required to initiate this process, the intracellular folding
and assembly of the amino-terminal 28% of apoB (apoB28) was examined using several
criteria including nonreducing gel electrophoresis, sensitivity to dithiothreitol (DTT)-mediated
reduction, and buoyant density gradient centrifugation. In hepatoma cells, after a 1-min
pulse with radiolabeled amino acids, labeled apoB28 migrated during gel electrophoresis in …