[HTML][HTML] In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio)

GM Her, CC Chiang, WY Chen, JL Wu - FEBS letters, 2003 - Elsevier
GM Her, CC Chiang, WY Chen, JL Wu
FEBS letters, 2003Elsevier
Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various
tissues including liver, small intestine and kidney and is thought to play a crucial role in
intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific
regulation during zebrafish hepatogenesis, we isolated 5′-flanking sequences of the
zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to
generate liver-specific transgenic zebrafish. The 2.8-kb 5′-flanking sequence of zebrafish L …
Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5′-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5′-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42–51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development.
Elsevier