Single-tier testing with the C6 peptide ELISA kit compared with two-tier testing for Lyme disease
GP Wormser, M Schriefer, ME Aguero-Rosenfeld… - … and infectious disease, 2013 - Elsevier
For the diagnosis of Lyme disease, the 2-tier serologic testing protocol for Lyme disease has
a number of shortcomings including low sensitivity in early disease; increased cost, time,
and labor; and subjectivity in the interpretation of immunoblots. In this study, the diagnostic
accuracy of a single-tier commercial C6 ELISA kit was compared with 2-tier testing. The
results showed that the C6 ELISA was significantly more sensitive than 2-tier testing with
sensitivities of 66.5%(95% confidence interval [CI] 61.7–71.1) and 35.2%(95% CI 30.6 …
a number of shortcomings including low sensitivity in early disease; increased cost, time,
and labor; and subjectivity in the interpretation of immunoblots. In this study, the diagnostic
accuracy of a single-tier commercial C6 ELISA kit was compared with 2-tier testing. The
results showed that the C6 ELISA was significantly more sensitive than 2-tier testing with
sensitivities of 66.5%(95% confidence interval [CI] 61.7–71.1) and 35.2%(95% CI 30.6 …
