Long-term culture of murine bone marrow precursors of B lymphocytes
CA Whitlock, ON Witte - Methods in enzymology, 1987 - Elsevier
Publisher Summary This chapter describes a method for preparing murine bone marrow and
fetal liver cultures that selectively support B lymphopoiesis. The techniques for initiation and
maintenance are simple but require careful attention to a few key parameters. The age and
strain of mice used as a source of bone marrow and the batch of fetal calf serum all
determine the efficiency with which cultures establish. A method for obtaining larger
numbers of cells by expanding the cultures onto adherent feeder cell layers is provided. The …
fetal liver cultures that selectively support B lymphopoiesis. The techniques for initiation and
maintenance are simple but require careful attention to a few key parameters. The age and
strain of mice used as a source of bone marrow and the batch of fetal calf serum all
determine the efficiency with which cultures establish. A method for obtaining larger
numbers of cells by expanding the cultures onto adherent feeder cell layers is provided. The …
