Preferential recruitment of CCR6-expressing Th17 cells to inflamed joints via CCL20 in rheumatoid arthritis and its animal model

K Hirota, H Yoshitomi, M Hashimoto, S Maeda… - The Journal of …, 2007 - rupress.org
K Hirota, H Yoshitomi, M Hashimoto, S Maeda, S Teradaira, N Sugimoto, T Yamaguchi
The Journal of experimental medicine, 2007rupress.org
This report shows that interleukin (IL) 17–producing T helper type 17 (Th17) cells
predominantly express CC chemokine receptor (CCR) 6 in an animal model of rheumatoid
arthritis (RA). Th17 cells induced in vivo in normal mice via homeostatic proliferation
similarly express CCR6, whereas those inducible in vitro by transforming growth factor β and
IL-6 additionally need IL-1 and neutralization of interferon (IFN) γ and IL-4 for CCR6
expression. Forced expression of RORγt, a key transcription factor for Th17 cell …
This report shows that interleukin (IL) 17–producing T helper type 17 (Th17) cells predominantly express CC chemokine receptor (CCR) 6 in an animal model of rheumatoid arthritis (RA). Th17 cells induced in vivo in normal mice via homeostatic proliferation similarly express CCR6, whereas those inducible in vitro by transforming growth factor β and IL-6 additionally need IL-1 and neutralization of interferon (IFN) γ and IL-4 for CCR6 expression. Forced expression of RORγt, a key transcription factor for Th17 cell differentiation, induces not only IL-17 but also CCR6 in naive T cells. Furthermore, Th17 cells produce CCL20, the known ligand for CCR6. Synoviocytes from arthritic joints of mice and humans also produce a large amount of CCL20, with a significant correlation (P = 0.014) between the amounts of IL-17 and CCL20 in RA joints. The CCL20 production by synoviocytes is augmented in vitro by IL-1β, IL-17, or tumor necrosis factor α, and is suppressed by IFN-γ or IL-4. Administration of blocking anti-CCR6 monoclonal antibody substantially inhibits mouse arthritis. Thus, the joint cytokine milieu formed by T cells and synovial cells controls the production of CCL20 and, consequently, the recruitment of CCR6+ arthritogenic Th17 cells to the inflamed joints. These results indicate that CCR6 expression contributes to Th17 cell function in autoimmune disease, especially in autoimmune arthritis such as RA.
rupress.org