High-throughput analysis of 19 endogenous androgenic steroids by ultra-performance convergence chromatography tandem mass spectrometry

JL Quanson, MA Stander, E Pretorius… - … of Chromatography B, 2016 - Elsevier
Oxygenated steroids such as 11-ketotestosterone and 11-ketodihydrotestosterone have
recently been shown to play a putative role in the development and progression of castration
resistant prostate cancer. In this study we report on the development of a high throughput
ultra-performance convergence chromatography tandem mass spectrometry (UPC 2-
MS/MS) method for the analysis of thirteen 11-oxygenated and six canonical C19 steroids
isolated from a cell culture matrix. Using an Acquity UPC 2 BEH 2-EP column we found that …