Folding and assembly of newly synthesized thyroglobulin occurs in a pre-Golgi compartment
PS Kim, P Arvan - Journal of Biological Chemistry, 1991 - ASBMB
We have investigated the kinetics of folding and dimerization of newly synthesized
thyroglobulin (Tg), the precursor protein in the manufacture of thyroid hormone. From the
examination of lysates of pulse-labeled cultured thyrocytes by denaturing and
nondenaturing gel electrophoresis, we have found that the earliest detectable form of
nascent Tg is a transient aggregate, whose dissolution in vitro requires the addition of a
reducing agent. In vivo, aggregate dissolution occurs with a t1/2 approximately 10 min at 37 …
thyroglobulin (Tg), the precursor protein in the manufacture of thyroid hormone. From the
examination of lysates of pulse-labeled cultured thyrocytes by denaturing and
nondenaturing gel electrophoresis, we have found that the earliest detectable form of
nascent Tg is a transient aggregate, whose dissolution in vitro requires the addition of a
reducing agent. In vivo, aggregate dissolution occurs with a t1/2 approximately 10 min at 37 …