[HTML][HTML] Cis and trans actions of the cholinesterase-like domain within the thyroglobulin dimer
Thyroglobulin (Tg, precursor for thyroid hormone synthesis) is a large secreted glycoprotein
composed of upstream regions I-II-III, followed by the∼ 570 residue cholinesterase-like
(ChEL) domain. ChEL has two identified functions: 1) homodimerization, and 2) binding to I-
II-III that facilitates I-II-III oxidative maturation required for intracellular protein transport. Like
its homologs in the acetylcholinesterase (AChE) family, ChEL possesses two carboxyl-
terminal α-helices. We find that a Tg-AChE chimera (swapping AChE in place of ChEL) …
composed of upstream regions I-II-III, followed by the∼ 570 residue cholinesterase-like
(ChEL) domain. ChEL has two identified functions: 1) homodimerization, and 2) binding to I-
II-III that facilitates I-II-III oxidative maturation required for intracellular protein transport. Like
its homologs in the acetylcholinesterase (AChE) family, ChEL possesses two carboxyl-
terminal α-helices. We find that a Tg-AChE chimera (swapping AChE in place of ChEL) …